Microarrays (DNA Chips) for the Classroom Laboratory

Autor/inn/en	Barnard, Betsy; Sussman, Michael; BonDurant, Sandra Splinter; Nienhuis, James; Krysan, Patrick
Titel	Microarrays (DNA Chips) for the Classroom Laboratory
Quelle	In: Biochemistry and Molecular Biology Education, 34 (2006) 5, S. 355-359Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.2006.494034052645
Schlagwörter	Leitfaden; Lehrerheft; Forschungsbericht; Secondary School Teachers; Research Tools; Laboratories; Botany; Genetics; Wisconsin + Suchen Sie Ihr Suchwort? Forschungsmittel; Laboratory; Laboratorium; Botanik; Humangenetik
Abstract	We have developed and optimized the necessary laboratory materials to make DNA microarray technology accessible to all high school students at a fraction of both cost and data size. The primary component is a DNA chip/array that students "print" by hand and then analyze using research tools that have been adapted for classroom use. The primary adaptation is the use of a simulated cDNA target. The low density DNA array we discuss here was used to demonstrate differential expression of several "Arabidopsis thaliana" genes related to photosynthesis and photomorphogenesis. The methods we present here can be used with any biological organism whose sequence is known. Furthermore, these methods can be adapted to exhibit a variety of differential gene expression patterns under different experimental conditions. The materials and tools we discuss have been applied in classrooms at West High School in Madison, WI. We have also shared these materials with high school teachers attending professional development courses at the University of Wisconsin-Madison. (Author).
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Begutachtung	Peer reviewed
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10