Unnatural Chemical Biology: Research-Based Laboratory Course Utilizing Genetic Code Expansion

Autor/inn/en	Kean, Kelsey M.; van Zee, Kari; Mehl, Ryan A.
Titel	Unnatural Chemical Biology: Research-Based Laboratory Course Utilizing Genetic Code Expansion
Quelle	In: Journal of Chemical Education, 96 (2019) 1, S. 66-74Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Mehl, Ryan A.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.8b00011
Schlagwörter	Forschungsbericht; Science Instruction; Biochemistry; Science Laboratories; Genetics; Organic Chemistry; Microbiology; Molecular Biology; Hands on Science; Inquiry; College Science; Undergraduate Study + Suchen Sie Ihr Suchwort? Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Biochemie; Humangenetik; Organische Chemie; Mikrobiologie; Molekularbiologie; Grundstudium
Abstract	The content and design scheme for a readily adaptable, research-based laboratory course in chemical biology are presented. In this course, students interrogate protein structure and function using the site-specific incorporation of noncanonical amino acids by genetic code expansion. The relatively new field of genetic code expansion enables protein engineering with a diverse array of chemical functional groups. In this quarter or semester research-based undergraduate laboratory experience, student teams design, synthesize, and evaluate the structure-function relationships of proteins containing noncanonical amino acids on the basis of a self-selected hypothesis and then communicate their results in a formal manuscript and research presentation. The flexibility and versatility of genetic code expansion and this course structure empower students to engineer novel biomolecules and highlight the use of organic-chemistry principles for interrogating proteins. Generating noncanonical protein variants that have never been produced previously exposes students to novel research and trains them in essential basic biochemistry skills. This research-based undergraduate laboratory course can easily be adapted, scaled, and implemented on the basis of the interests, demographics, and resources of a given institution. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Begutachtung	Peer reviewed
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01