Surface Plasmon Resonance Spectroscopy: A Versatile Technique in a Biochemist's Toolbox

Autor/in	Bakhtiar, Ray
Titel	Surface Plasmon Resonance Spectroscopy: A Versatile Technique in a Biochemist's Toolbox
Quelle	In: Journal of Chemical Education, 90 (2013) 2, S. 203-209Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/ed200549g
Schlagwörter	Forschungsbericht; Chemistry; Science Instruction; Spectroscopy; Biochemistry; Molecular Biology; College Science; Hands on Science; Scientific Concepts; Light; Computer Software; Educational Technology; Graduate Students; Undergraduate Students; Laboratory Experiments; Laboratory Equipment + Suchen Sie Ihr Suchwort? Chemie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Spektroskopie; Biochemie; Molekularbiologie; Licht; Unterrichtsmedien; Graduate Study; Student; Students; Aufbaustudium; Graduiertenstudium; Hauptstudium; Studentin; Laboratory work; Laborarbeit; Laborausstattung
Abstract	Surface plasmon resonance (SPR) spectroscopy is a powerful, label-free technique to monitor noncovalent molecular interactions in real time and in a noninvasive fashion. As a label-free assay, SPR does not require tags, dyes, or specialized reagents (e.g., enzymes-substrate complexes) to elicit a visible or a fluorescence signal. During the last two decades, SPR has been broadly applied to study of noncovalent interactions of protein-DNA, protein-cell, RNA-DNA, DNA-DNA, protein-protein, protein-carbohydrate, small molecule-macromolecule (e.g., receptor-inhibitor complex), protein-peptide, and self-assembled monolayers. In addition, SPR has been successfully applied to drug discovery ligand-fishing and clinical immunogenicity studies (i.e., to monitor an immune response against a therapeutic agent). SPR spectroscopy can address questions such as specificity of an interaction, kinetics, affinity, and concentrations of selected molecules present in a sample of interest. Given the current enhancements in hardware and software capabilities along with its ease of use and maintenance, SPR experiments can be designed for upper-level undergraduate biochemistry, biophysics, and physical chemistry laboratory courses. In this article, an overview of SPR phenomenon, instrumentation, sensor immobilization, and its selected applications is presented. (Contains 2 tables and 5 figures.) (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Begutachtung	Peer reviewed
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10