A Simple Microscopy Assay to Teach the Processes of Phagocytosis and Exocytosis

Autor/inn/en	Gray, Ross; Gray, Andrew; Fite, Jessica L.; Jordan, Renee; Stark, Sarah; Naylor, Kari
Titel	A Simple Microscopy Assay to Teach the Processes of Phagocytosis and Exocytosis
Quelle	In: CBE - Life Sciences Education, 11 (2012) 2, S.180-186 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1931-7913
DOI	10.1187/cbe.11-07-0060
Schlagwörter	Educational Games; Botany; Cytology; Science Process Skills; Teaching Methods; College Science; Undergraduate Students; Experiments; Data Analysis; Inquiry; Classroom Environment; Instructional Effectiveness + Suchen Sie Ihr Suchwort? Educational game; Lernspiel; Botanik; Zytologie; Teaching method; Lehrmethode; Unterrichtsmethode; Erprobung; Auswertung; Klassenklima; Unterrichtsklima; Unterrichtserfolg
Abstract	Phagocytosis and exocytosis are two cellular processes involving membrane dynamics. While it is easy to understand the purpose of these processes, it can be extremely difficult for students to comprehend the actual mechanisms. As membrane dynamics play a significant role in many cellular processes ranging from cell signaling to cell division to organelle renewal and maintenance, we felt that we needed to do a better job of teaching these types of processes. Thus, we developed a classroom-based protocol to simultaneously study phagocytosis and exocytosis in "Tetrahymena pyriformis." In this paper, we present our results demonstrating that our undergraduate classroom experiment delivers results comparable with those acquired in a professional research laboratory. In addition, students performing the experiment do learn the mechanisms of phagocytosis and exocytosis. Finally, we demonstrate a mathematical exercise to help the students apply their data to the cell. Ultimately, this assay sets the stage for future inquiry-based experiments, in which the students develop their own experimental questions and delve deeper into the mechanisms of phagocytosis and exocytosis. (Contains 4 figures and 2 tables.) (As Provided).
Anmerkungen	American Society for Cell Biology. 8120 Woodmont Avenue Suite 750, Bethesda, MD 20814-2762. Tel: 301-347-9300; Fax: 301-347-9310; e-mail: ascbinfo@ascb.org; Website: http://www.ascb.org
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10