Gene Expression Analysis: Teaching Students to Do 30,000 Experiments at Once with Microarray

Autor/inn/en	Carvalho, Felicia I.; Johns, Christopher; Gillespie, Marc E.
Titel	Gene Expression Analysis: Teaching Students to Do 30,000 Experiments at Once with Microarray
Quelle	In: Biochemistry and Molecular Biology Education, 40 (2012) 3, S.181-190 (10 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.20588
Schlagwörter	Genetics; Science Experiments; Laboratory Experiments; College Science; Science Instruction; Undergraduate Students + Suchen Sie Ihr Suchwort? Humangenetik; Laboratory work; Laborarbeit; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht
Abstract	Genome scale experiments routinely produce large data sets that require computational analysis, yet there are few student-based labs that illustrate the design and execution of these experiments. In order for students to understand and participate in the genomic world, teaching labs must be available where students generate and analyze large data sets. We present a microarray-based gene expression analysis experiment that is tailored for undergraduate students. The methods in this article describe an expression analysis experiment that can also be applied to CGH and SNP experiments. Factors such as technical difficulty, duration, cost, and availability of materials and equipments are considered in the lab design. The microarray teaching lab is performed in two sessions. The first is an introductory wet bench exercise that allows students to master the basic technical skills. The second builds on the concepts and skills with students acquiring and analyzing the microarray data. This lab exercise familiarizes students with large-scale data experiments and introduces them to the initial analysis steps. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10