Mapping Maize Genes: A Series of Research-Based Laboratory Exercises

Autor/inn/en	Makarevitch, Irina; Kralich, Elizabeth
Titel	Mapping Maize Genes: A Series of Research-Based Laboratory Exercises
Quelle	In: Biochemistry and Molecular Biology Education, 39 (2011) 5, S.367-375 (9 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.20524
Schlagwörter	Genetics; Laboratory Experiments; Science Activities; Science Course Improvement Projects; Problem Sets; Concept Mapping; Concept Teaching; Food; Biotechnology; Biochemistry; Molecular Biology; Teaching Methods; Undergraduate Students; College Science; Scientific Concepts + Suchen Sie Ihr Suchwort? Humangenetik; Laboratory work; Laborarbeit; Problemstellung; Concept Map; Lebensmittel; Biotechnologie; Biochemie; Molekularbiologie; Teaching method; Lehrmethode; Unterrichtsmethode
Abstract	Open-ended, inquiry-based multiweek laboratory exercises are the key elements to increasing students' understanding and retention of the major biological concepts. Including original research into undergraduate teaching laboratories has also been shown to motivate students and improve their learning. Here, we present a series of original laboratory exercises on fine mapping novel maize mutations producing interesting phenotypes. In this 4-week lab series, students get involved in the whole process of identifying novel genes controlling specific phenotypes, from phenotype characterization and choosing appropriate molecular markers to calculating the genetic distance between the mutation and the marker and finding possible candidate genes using a complete genome sequence. We chose to use maize mutant lines produced by TILLING project. These lines have been partially mapped to a chromosomal bin by a high-throughput bulk segregant analysis; however, the exact map positions for these mutations have never been determined. Mapping these novel maize mutations provides students with the opportunity to conduct original research as a part of their classroom experience and to contribute to the field of maize genetics. The laboratory series was well received by the students, and the assessment results demonstrated an improvement of student learning of gene mapping, molecular marker analysis, and positional cloning concepts. (Contains 4 figures and 5 tables.) (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10