An "in Silico" DNA Cloning Experiment for the Biochemistry Laboratory

Autor/in	Elkins, Kelly M.
Titel	An "in Silico" DNA Cloning Experiment for the Biochemistry Laboratory
Quelle	In: Biochemistry and Molecular Biology Education, 39 (2011) 3, S.211-215 (5 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.20466
Schlagwörter	Biochemistry; Molecular Biology; Laboratory Experiments; Science Experiments; Genetics; Undergraduate Students; College Science; Science Instruction; Biotechnology; Advanced Courses; Student Attitudes; Science Activities + Suchen Sie Ihr Suchwort? Biochemie; Molekularbiologie; Laboratory work; Laborarbeit; Humangenetik; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Biotechnologie; Fortgeschrittenenunterricht; Schülerverhalten
Abstract	This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced high school biology classes. Students begin by examining a plasmid map with the goal of identifying which restriction enzymes may be used to clone a piece of foreign DNA containing a gene of interest into the vector. From the National Center for Biotechnology Initiative website, students are instructed to retrieve a protein sequence and use Expasy's Reverse Translate program to reverse translate the protein to cDNA. Students then use Integrated DNA Technologies' OligoAnalyzer to predict the complementary DNA strand and obtain DNA recognition sequences for the desired restriction enzymes from New England Biolabs' website. Students add the appropriate DNA restriction sequences to the double-stranded foreign DNA for cloning into the plasmid and infecting "Escherichia coli" cells. Students are introduced to computational biology tools, molecular biology terminology and the process of DNA cloning in this valuable single session, "in silico" experiment. This project develops students' understanding of the cloning process as a whole and contrasts with other laboratory and internship experiences in which the students may be involved in only a piece of the cloning process/techniques. Students interested in pursuing postgraduate study and research or employment in an academic biochemistry or molecular biology laboratory or industry will benefit most from this experience. (Contains 1 figure and 2 tables.) (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10