A Simple Assay to Quantify Metallothionein Helps to Learn about Bioindicators and Environmental Health

Autor/inn/en	Linde, Ana R.; Garcia-Vazquez, Eva
Titel	A Simple Assay to Quantify Metallothionein Helps to Learn about Bioindicators and Environmental Health
Quelle	In: Biochemistry and Molecular Biology Education, 34 (2006) 5, S.360-363 (4 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.2006.494034052653
Schlagwörter	Leitfaden; Unterricht; Lehrer; Foreign Countries; Natural Resources; Laboratories; Public Health; Chemistry; Molecular Biology; Hands on Science; Environmental Influences; Environmental Education; Environmental Research; Animals; Pollution; Ecology; Spectroscopy; Brazil; Spain + Suchen Sie Ihr Suchwort? Lesson concept; Instruction; Unterrichtsentwurf; Unterrichtsprozess; Teacher; Teachers; Lehrerin; Lehrende; Ausland; Natural Ressource; Natürliche Ressource; Laboratory; Laboratorium; Gesundheitswesen; Chemie; Molekularbiologie; Environmental influence; Umwelteinfluss; Umweltbildung; Umwelterziehung; Umweltpädagogik; Environmental study; Umweltforschung; Animal; Tier; Tiere; Schadstoffbelastung; Ökologie; Spektroskopie; Brasilien; Spanien
Abstract	We present a laboratory practice aimed to clarify and discuss the concept of bioindicator molecules in environmental health. It has been implemented as a hands-on laboratory exercise in postgraduate courses in public health (in Brazil) and in a course entitled Biology Applied to Natural Resources Sustainability (in Spain) but can be useful for undergraduate studies. By simple, repeatable, and low cost spectrophotometry methodology, students detect minimal concentrations (nmol) of metallothionein in biological samples obtained from sites with various status of pollution. They can also expose experimental fish or mollusks to heavy metals in aquarium conditions and then detect metallothionein. This method is excellent for didactic purposes because it can be developed even in modest laboratories with basic equipment for molecular biology. (Author).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10