Identification of Highly Prized Commercial Fish Using a PCR-Based Methodology

Autor/inn/en	Moran, Paloma; Garcia-Vasquez, Eva
Titel	Identification of Highly Prized Commercial Fish Using a PCR-Based Methodology
Quelle	In: Biochemistry and Molecular Biology Education, 34 (2006) 2, S.121-124 (4 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.2006.49403402121
Schlagwörter	Leitfaden; Unterricht; Lehrer; Undergraduate Students; Identification; Molecular Biology; Animals; Genetics; Marine Biology; Science Instruction; Teaching Methods + Suchen Sie Ihr Suchwort? Lesson concept; Instruction; Unterrichtsentwurf; Unterrichtsprozess; Teacher; Teachers; Lehrerin; Lehrende; Identifikation; Identifizierung; Molekularbiologie; Animal; Tier; Tiere; Humangenetik; Meeresbiologie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Teaching method; Lehrmethode; Unterrichtsmethode
Abstract	We report a practical class designed for undergraduate students of Marine Sciences as a part of the Genetics course. The class can also be included in undergraduate studies of food technology. The exercise was designed to emphasize the application of molecular biology techniques to fish species authentication and traceability. After a simple and rapid protocol for DNA extraction, PCR was used to analyze variation in size of individual "5S" ribosomal gene spacer sequences in eight commercially important fishes. The resulting fragments containing these sequences were separated on agarose gels and visualized under a UV lamp. Differences in fragment length can be used to differentiate between species. This method is particularly useful in the identification of larvae, eggs, and processed food including frozen and canned products. (Author).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10