An Undergraduate Laboratory to Detect Viruses in Human DNA Samples Using qPCR

Autor/inn/en	Militello, Kevin T.; Nedelkovska, Hristina
Titel	An Undergraduate Laboratory to Detect Viruses in Human DNA Samples Using qPCR
Quelle	In: Biochemistry and Molecular Biology Education, 50 (2022) 3, S.319-325 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Militello, Kevin T.) ORCID (Nedelkovska, Hristina)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21620
Schlagwörter	Genetics; Undergraduate Students; Patients; Accuracy; COVID-19; Pandemics; Diagnostic Tests; Comparative Analysis; Data Interpretation; Laboratory Experiments; Science Tests; Student Attitudes; Positive Attitudes; Instructional Effectiveness; Teaching Methods + Suchen Sie Ihr Suchwort? Humangenetik; Patient; Diagnostic test; Diagnostischer Test; Data evaluation; Datenauswertung; Laboratory work; Laborarbeit; Schülerverhalten; Unterrichtserfolg; Teaching method; Lehrmethode; Unterrichtsmethode
Abstract	The COVID-19 pandemic has necessitated the need to reliably detect the presence of viral genomes in human clinical samples. The most accurate viral tests involve the use of qPCR. Thus, it is important for students to understand the mechanism to detect viral genomes by qPCR including critical qPCR controls and how to properly interpret qPCR data. Herein, we describe a 2-week undergraduate laboratory to detect a viral genome in a human DNA sample. The strategy follows a SARS-CoV-2 qPCR test in numerous ways. Students are provided isolated DNA representing a mock human patient sample, and determine if a viral genome (bacteriophage lambda) is present using qPCR. A battery of control samples and patient pooling strategies are utilized. The laboratory exercise is successful based on high rates of student success in detecting viral genomes, pre-quiz and post-quiz assessments focusing on viral qPCR testing, and positive student comments. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01