An Integrated Laboratory Experiment for the Determination of Main Components in Different Food Samples

Autor/inn/en	Khalid, Habiba; Amin, Farrukh Raza; Chen, Chang
Titel	An Integrated Laboratory Experiment for the Determination of Main Components in Different Food Samples
Quelle	In: Biochemistry and Molecular Biology Education, 50 (2022) 1, S.133-141 (9 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Chen, Chang)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21592
Schlagwörter	Laboratory Experiments; Science Experiments; Introductory Courses; Biochemistry; Scientific Concepts; Food; College Students; Hands on Science; Scientific Research; Biological Sciences + Suchen Sie Ihr Suchwort? Laboratory work; Laborarbeit; Einführungskurs; Biochemie; Lebensmittel; Collegestudent; Abwasserbiologie
Abstract	An integrated laboratory experiment was designed for introducing biochemistry students to basic static biochemistry to deepen their understanding on the properties and analysis of biomolecules such as total carbohydrates, lipid, protein, and protein-constituent amino acids. Food represents a very important source of biomolecules of technological and functional interest; therefore, 15 types of food samples were selected to demonstrate the analysis of basic composition of these biomolecules. In this experiment, students learnt testing the total carbohydrates of all the food samples using 3,5-dinitrosalicylic acid (DNS) method, while performing acid hydrolysis. Then, lipid extraction was done using Soxhlet extraction method in order to determine the crude lipid concentration in different samples. After this, the students learnt testing crude protein content of these samples by using Kjeldahl method, and amino acid analysis was performed using HPLC. From the experiments, students grasped the concept and advantages of these methods and deepened their understanding on compositional analysis of different food samples. This laboratory exercise can be included into any college-level biochemistry courses and gives hands-on experience to the students for conducting scientific research in the field of life sciences, food science, and other bio-related fields. (As Provided).
Anmerkungen	Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01