Literaturnachweis - Detailanzeige
| Autor/inn/en | Crane, Julia; Page, Shallee T. |
|---|---|
| Titel | An Undergraduate Laboratory Experiment with Real-World Applications: Utilizing Templateless Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction to Test for SARS-CoV-2 RNA |
| Quelle | In: Biochemistry and Molecular Biology Education, 50 (2022) 1, S.142-148 (7 Seiten)Infoseite zur Zeitschrift
PDF als Volltext |
| Zusatzinformation | ORCID (Page, Shallee T.) |
| Sprache | englisch |
| Dokumenttyp | gedruckt; online; Zeitschriftenaufsatz |
| ISSN | 1470-8175 |
| DOI | 10.1002/bmb.21593 |
| Schlagwörter | Undergraduate Students; College Science; Science Instruction; Scientific Research; Laboratory Experiments; Genetics; COVID-19; Microbiology; Molecular Biology; Biochemistry; Biotechnology |
| Abstract | We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published protocols, we assembled a successful project that illustrated a topical exercise similar to real-world assay development. The exercise is aimed at upper-level undergraduates and requires 3 weeks of laboratory periods. The students were able to design and test experimental protocols, while learning about RNA detection. This project could be utilized in upper-level classes including molecular biology, biochemistry, biotechnology, or for independent research projects. (As Provided). |
| Anmerkungen | Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us |
| Erfasst von | ERIC (Education Resources Information Center), Washington, DC |
| Update | 2024/1/01 |
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