A Guided-Inquiry Investigation of Genetic Variants Using Oxford Nanopore Sequencing for an Undergraduate Molecular Biology Laboratory Course

Autor/inn/en	Rodriguez, Princess D.; Mariani, Michael; Gay, Jamie; Hogan, Tyler C.; Amiel, Eyal; Deming, Paula B.; Frietze, Seth
Titel	A Guided-Inquiry Investigation of Genetic Variants Using Oxford Nanopore Sequencing for an Undergraduate Molecular Biology Laboratory Course
Quelle	In: Biochemistry and Molecular Biology Education, 49 (2021) 4, S.588-597 (10 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Frietze, Seth)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21514
Schlagwörter	Molecular Biology; Science Instruction; Teaching Methods; Undergraduate Students; Cancer; Cytology; Inquiry; Laboratory Experiments; Genetics; Identification; Profiles; Student Attitudes; Self Concept; Scientific Concepts; Concept Formation + Suchen Sie Ihr Suchwort? Molekularbiologie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Teaching method; Lehrmethode; Unterrichtsmethode; Carcinoma; Karzinom; Krebs (med); Krebserkrankung; Zytologie; Laboratory work; Laborarbeit; Humangenetik; Identifikation; Identifizierung; Charakterisierung; Profilanalyse; Schülerverhalten; Selbstkonzept; Concept learning; Begriffsbildung
Abstract	Next Generation Sequencing (NGS) has become an important tool in the biological sciences and has a growing number of applications across medical fields. Currently, few undergraduate programs provide training in the design and implementation of NGS applications. Here, we describe an inquiry-based laboratory exercise for a college-level molecular biology laboratory course that uses real-time MinION deep sequencing and bioinformatics to investigate characteristic genetic variants found in cancer cell-lines. The overall goal for students was to identify non-small cell lung cancer (NSCLC) cell-lines based on their unique genomic profiles. The units described in this laboratory highlight core principles in multiplex PCR primer design, real-time deep sequencing, and bioinformatics analysis for genetic variants. We found that the MinION device is an appropriate, feasible tool that provides a comprehensive, hands-on NGS experience for undergraduates. Student evaluations demonstrated increased confidence in using molecular techniques and enhanced understanding of NGS concepts. Overall, this exercise provides a pedagogical tool for incorporating NGS approaches in the teaching laboratory as way of enhancing students' comprehension of genomic sequence analysis. Further, this NGS lab module can easily be added to a variety of lab-based courses to help undergraduate students learn current DNA sequencing methods with limited effort and cost. (As Provided).
Anmerkungen	Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01