Utilizing Loop-Mediated Isothermal Amplification to Detect the Presence of "Escherichia coli": An Inquiry-Driven Undergraduate Laboratory Module

Autor/inn/en	Roy, Brandon G.; Ryndock, Eric; Lappas, Courtney M.
Titel	Utilizing Loop-Mediated Isothermal Amplification to Detect the Presence of "Escherichia coli": An Inquiry-Driven Undergraduate Laboratory Module
Quelle	In: Biochemistry and Molecular Biology Education, 49 (2021) 3, S.475-482 (8 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Roy, Brandon G.) ORCID (Lappas, Courtney M.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21460
Schlagwörter	Undergraduate Students; College Science; Science Laboratories; Laboratory Experiments; Molecular Biology; Immunization Programs; Microbiology; Genetics; Scientific Concepts; Scientific Methodology; Science Process Skills; Evaluative Thinking; Data Analysis + Suchen Sie Ihr Suchwort? Laboratory work; Laborarbeit; Molekularbiologie; Immunisierung; Mikrobiologie; Humangenetik; Auswertung
Abstract	The amplification of nucleic acids is a fundamental tool utilized in various scientific disciplines, including Molecular Biology, Immunology, Microbiology, and Genetics. However, due to the time and technology required for traditional polymerase chain reaction and its derivatives, it is not always possible to include such methodologies in undergraduate laboratory curricula. Loop-mediated isothermal amplification (LAMP), a technology that has become increasingly utilized in a variety of laboratory and field settings during the past two decades, is an alternate method of nucleic acid amplification that is rapid, sensitive, and performed under isothermal conditions. We describe an adaptable, inquiry-driven laboratory module that is focused on the detection of "Escherichia coli" DNA via LAMP amplification. The main objectives of the module are to introduce students to the principles and protocols of LAMP, to help students develop the ability to apply the scientific method to scientific questions, to guide students as they develop the ability to identify the most appropriate methodology to use in the investigation of scientific questions, and to train students to critically evaluate scientific data, theories, and principles and to articulate their evaluations in both written and oral formats. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01