Literaturnachweis - Detailanzeige
Autor/inn/en | Sharma, Kanika; Fizet, Kiel J.; Montgomery, Kathlyene R.; Smeltzer, Nathan A.; Sikorski, Madison H.; Brown, Kennedy G.; Beyke, Brandon J.; Burkhart, Ryan C.; Lynn, Abigail N.; Grandinetti, Giovanna |
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Titel | A Simple Colorimetric Experiment Using Mammalian Cell Culture to Study Metabolism |
Quelle | In: Biochemistry and Molecular Biology Education, 49 (2021) 2, S.271-277 (7 Seiten)Infoseite zur Zeitschrift
PDF als Volltext |
Zusatzinformation | ORCID (Grandinetti, Giovanna) |
Sprache | englisch |
Dokumenttyp | gedruckt; online; Zeitschriftenaufsatz |
ISSN | 1470-8175 |
DOI | 10.1002/bmb.21457 |
Schlagwörter | Biochemistry; Cytology; Metabolism; Science Laboratories; Laboratory Procedures; Undergraduate Students; Laboratory Experiments; Pharmacology; Animals |
Abstract | The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In this experiment, students are tasked with relating the acidification of cell culture medium to metabolism in order to elucidate the mechanism of action for a compound. Students can relate their experimental results to topics covered on glycolysis and oxidative phosphorylation in upper-level biochemistry classes as well as gain valuable experience relating metabolism to drug discovery. (As Provided). |
Anmerkungen | Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us |
Erfasst von | ERIC (Education Resources Information Center), Washington, DC |
Update | 2024/1/01 |