A Laboratory Practice That Uses the Polymerase Chain Reaction-Sequence Specific Priming Technique to Rapidly Screen for HLA-DR2 Allotype from Germline DNA in Immunology Course for Undergraduate Medical Students

Autor/inn/en	Li, LiXin; Tian, Wei; Zhang, Jie
Titel	A Laboratory Practice That Uses the Polymerase Chain Reaction-Sequence Specific Priming Technique to Rapidly Screen for HLA-DR2 Allotype from Germline DNA in Immunology Course for Undergraduate Medical Students
Quelle	In: Biochemistry and Molecular Biology Education, 48 (2020) 2, S.175-180 (6 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Tian, Wei)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21322
Schlagwörter	Undergraduate Students; Medical Students; Molecular Biology; Genetics; Laboratory Experiments; Biochemistry + Suchen Sie Ihr Suchwort? Molekularbiologie; Humangenetik; Laboratory work; Laborarbeit; Biochemie
Abstract	In this article, we describe an in-house polymerase chain reaction-sequence specific priming (PCR-SSP) assay designed for undergraduate medical students as part of the experimental pathogen biology and immunology (EPBI) course. It screens human leukocyte antigen (HLA)-DR2 allotype from genomic DNA samples using a rapid and single-tube PCR technique, yielding definitive typing result without conventional post-amplification step like probing or Sanger sequencing. This laboratory exercise offers the undergraduate medical students an opportunity to learn about current molecular biology techniques in HLA genotyping with limited effort and cost, in addition to a better understanding of concepts presented in the classroom lectures. Upon completing this experiment module, the students show statistically significant improvement in several key indexes, such as the knowledge about the mainstream HLA DNA typing techniques, awareness of the relevance of this knowledge for their future scientific research, immunogenetics-related basic laboratory skills they acquire, and interest and desire for mastering this assay (all p < 0.05). This easy to implement set of experiments is composed of a two-session lab module occupying eight teaching hours, and has been run successfully in our laboratory. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01