Harnessing Single-Stranded DNA Binding Protein to Explore Protein-Protein and Protein-DNA Interactions

Autor/inn/en	Chen, Stefanie H.; Goller, Carlos C.
Titel	Harnessing Single-Stranded DNA Binding Protein to Explore Protein-Protein and Protein-DNA Interactions
Quelle	In: Biochemistry and Molecular Biology Education, 48 (2020) 2, S.181-190 (10 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Chen, Stefanie H.) ORCID (Goller, Carlos C.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21324
Schlagwörter	Genetics; Biochemistry; Cytology; Laboratory Safety; Student Evaluation; Grades (Scholastic); Self Esteem; Student Participation; Student Attitudes; Science Experiments; Science Instruction + Suchen Sie Ihr Suchwort? Humangenetik; Biochemie; Zytologie; Schulnote; Studentische Bewertung; Notenspiegel; Self-esteem; Selbstaufmerksamkeit; Schülermitarbeit; Schülermitwirkung; Studentische Mitbestimmung; Schülerverhalten; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht
Abstract	Proteins must interact with a variety of other cellular components to properly perform their functions. We have developed a series of five experiments based on the robust bacterial single-stranded DNA binding protein (SSB) to characterize both known and unknown protein-protein and protein-DNA interactions. Students work in groups to generate and process data from electrophoretic mobility shift assays (EMSA), yeast two-hybrid, far Western, chromatin immunoprecipitation (ChIP), and fluorescence microscopy experiments, including choosing a novel condition for each. A gamification approach was used to encourage student participation and laboratory safety. Student learning was assessed using pre- and post-surveys and course grade data. The results indicate a clear increase in both content knowledge and confidence in the topics presented. Ranking of course activities indicated that performing the hands-on laboratory exercises was the most valuable course component, and over half of the students would choose to take another course with a similar gamification component. Each of the five laboratory experiments can be performed in combination with each other or integrated separately into a related course, and the gamification structure can be applied to any course. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01