Exploring Ligand--Protein Interaction: A Laboratory Exercise on Herbicide Binding to Plasma Transport Protein

Autor/inn/en	Roslan, Amira Adlin; Tayyab, Saad
Titel	Exploring Ligand--Protein Interaction: A Laboratory Exercise on Herbicide Binding to Plasma Transport Protein
Quelle	In: Biochemistry and Molecular Biology Education, 47 (2019) 2, S.156-160 (5 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Tayyab, Saad)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21207
Schlagwörter	Science Laboratories; Science Instruction; Spectroscopy; Science Experiments; Laboratory Experiments; Undergraduate Students; College Science; Scientific Concepts + Suchen Sie Ihr Suchwort? Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Spektroskopie; Laboratory work; Laborarbeit
Abstract	A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM) concentrations produced a consecutive decrease in the protein's fluorescence. Treatment of the fluorescence quenching data according to the Stern--Volmer equation yielded the values of the Stern--Volmer constant (K[subscript sv]) and bimolecular quenching rate constant (k[subscript q]), whereas values of the binding constant (K[subscript a]) and number of binding sites (n) were obtained from the double logarithmic plot. This experiment provides an exciting opportunity for undergraduate students to independently perform ligand binding studies with a protein, in addition to providing the means for the determination of their binding parameters. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01