Analysis of Lactase in Lactose Intolerance Supplements

Autor/inn/en	Stourman, Nina; Moore, Jennifer
Titel	Analysis of Lactase in Lactose Intolerance Supplements
Quelle	In: Biochemistry and Molecular Biology Education, 46 (2018) 6, S.652-662 (11 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Stourman, Nina)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21185
Schlagwörter	Biochemistry; College Science; Science Laboratories; Inquiry; Laboratory Experiments; Science Experiments; Physiology; Metabolism; Concept Formation; Scientific Concepts; Student Research; Drug Therapy + Suchen Sie Ihr Suchwort? Biochemie; Laboratory work; Laborarbeit; Physiologie; Stoffwechsel; Concept learning; Begriffsbildung; Studentenforschung
Abstract	Lactase is the enzyme responsible for the digestion of the disaccharide lactose, and deficiency in this enzyme causes the prevalent medical condition lactose intolerance. Management of lactose intolerance can be achieved through the administration of lactase supplements. Lactase is an appropriate platform for advanced enzymatic study because its medical application is a motivator for student learning. The following is an upper-level biochemistry laboratory sequence that integrates student inquiry and exposure to advanced laboratory techniques. Students investigate three different lactase supplements through experimentation that includes the Bradford assay, SDS-PAGE, continuous and discontinuous kinetic assays, and zymography. Upon completion of this project, students compile their results and conclusions in a scientifically formatted paper comparing supplement protein content and activity. This safe and inexpensive laboratory project enriches student understanding of key biochemical concepts while mirroring work performed in a realistic research setting. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01