Aqueous Biphasic Systems in the Separation of Food Colorants

Autor/inn/en	Santos, João H. P. M.; Capela, Emanuel V.; Boal-Palheiros, Isabel; Coutinho, João A. P.; Freire, Mara G.; Ventura, Sónia P. M.
Titel	Aqueous Biphasic Systems in the Separation of Food Colorants
Quelle	In: Biochemistry and Molecular Biology Education, 46 (2018) 4, S.390-397 (8 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Santos, João H. P. M.) ORCID (Capela, Emanuel V.) ORCID (Coutinho, João A. P.) ORCID (Freire, Mara G.) ORCID (Ventura, Sónia P. M.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21125
Schlagwörter	Leitfaden; Unterricht; Lehrer; Science Instruction; Molecular Biology; Scientific Concepts; Teaching Methods; Food; Color; Science Experiments; Science Process Skills; Water; Hands on Science + Suchen Sie Ihr Suchwort? Lesson concept; Instruction; Unterrichtsentwurf; Unterrichtsprozess; Teacher; Teachers; Lehrerin; Lehrende; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Molekularbiologie; Teaching method; Lehrmethode; Unterrichtsmethode; Lebensmittel; Colour; Farbbezeichnung; Farbe; Wasser
Abstract	Aqueous biphasic systems (ABS) composed of polypropylene glycol and carbohydrates, two benign substances are proposed to separate two food colorants (E122 and E133). ABS are promising extractive platforms, particularly for biomolecules, due to their aqueous and mild nature (pH and temperature), reduced environmental impact and processing costs. Another major aspect considered, particularly useful in downstream processing, is the "tuning" ability for the extraction and purification of these systems by a proper choice of the ABS components. In this work, our intention is to show the concept of ABS as an alternative and volatile organic solvent-free tool to separate two different biomolecules in a simple way, so simple that teachers can effectively adopt it in their classes to explain the concept of bioseparation processes. Informative documents and general information about the preparation of binodal curves and their use in the partition of biomolecules is available in this work to be used by teachers in their classes. In this sense, the students use different carbohydrates to build ABS, then study the partition of two food color dyes (synthetic origin), thus evaluating their ability on the separation of both food colorants. Through these experiments, the students get acquainted with ABS, learn how to determine solubility curves and perform extraction procedures using colorant food additives, that can also be applied in the extraction of various (bio)molecules. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01