An Integrated Course Based on Two Dimensional-Electrophoresis and Mass Spectrometry Analysis to Teach Proteomic Techniques for Undergraduate Students

Autor/inn/en	Sha, Shanshan; Jia, Liqiu; Xin, Yi; Yao, Weili; Ma, Yufang
Titel	An Integrated Course Based on Two Dimensional-Electrophoresis and Mass Spectrometry Analysis to Teach Proteomic Techniques for Undergraduate Students
Quelle	In: Biochemistry and Molecular Biology Education, 46 (2018) 4, S.354-360 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Ma, Yufang)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21128
Schlagwörter	Spectroscopy; Undergraduate Students; College Science; Science Instruction; Science Experiments; Scientific Concepts; Concept Formation; Chemistry + Suchen Sie Ihr Suchwort? Spektroskopie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Concept learning; Begriffsbildung; Chemie
Abstract	Here, we developed an integrated course based on two dimensional-electrophoresis and spectrometry mass (2DE-MS) technique for undergraduate students to help them learn proteomic techniques. The soluble proteins in wild type and gene knockout bacteria were separated by 2DE and the differently expressed proteins were identified by MS analysis. The proteomic data was finally confirmed by RT-PCR detection. The separated experiments of 2DE, MS, RNA isolation, RT-PCR, as well as essential bioinformatic analysis, were integrated into a one-week course, which provided students an opportunity to systematically understand the proteomic techniques and their applications in current scientific research. (As Provided).
Anmerkungen	Wiley-Blackwell. 350 Main Street, Malden, MA 02148. Tel: 800-835-6770; Tel: 781-388-8598; Fax: 781-388-8232; e-mail: cs-journals@wiley.com; Web site: http://www.wiley.com/WileyCDA
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01