[superscript 31]P NMR of the Pyruvate Kinase Reaction: An Undergraduate Experiment in Enzyme Kinetics

Autor/inn/en	Werner, R. Marshall; Johnson, Austin
Titel	[superscript 31]P NMR of the Pyruvate Kinase Reaction: An Undergraduate Experiment in Enzyme Kinetics
Quelle	In: Biochemistry and Molecular Biology Education, 45 (2017) 6, S.509-514 (6 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Werner, R. Marshall)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21079
Schlagwörter	Undergraduate Students; Science Instruction; Biochemistry; Spectroscopy; Science Experiments; Teaching Methods + Suchen Sie Ihr Suchwort? Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Biochemie; Spektroskopie; Teaching method; Lehrmethode; Unterrichtsmethode
Abstract	Understanding how to perform an enzyme assay is a critical learning skill in the undergraduate biochemistry curriculum. Students in biochemistry typically have been exposed to the use of NMR spectroscopy as a tool to determine chemical structure, but rarely are they exposed to the utility of NMR to evaluate enzyme kinetics. Furthermore, coverage of NMR experiments utilizing "alternative nuclei," such as [superscript 15]N, [superscript 19]F, and [superscript 31]P may be neglected. Herein we report a simple 31P NMR tube experiment that allows students to examine the enzyme kinetics and equilibrium constant of the reaction catalyzed by pyruvate kinase. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01