Better Understanding of Homologous Recombination through a 12-Week Laboratory Course for Undergraduates Majoring in Biotechnology

Autor/inn/en	Li, Ming; Shen, Xiaodong; Zhao, Yan; Hu, Xiaomei; Hu, Fuquan; Rao, Xiancai
Titel	Better Understanding of Homologous Recombination through a 12-Week Laboratory Course for Undergraduates Majoring in Biotechnology
Quelle	In: Biochemistry and Molecular Biology Education, 45 (2017) 4, S.329-335 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21043
Schlagwörter	Undergraduate Students; Biotechnology; Majors (Students); Scientific Concepts; Molecular Biology; Genetics; Science Laboratories; Communicable Diseases; Science Experiments; Science Process Skills; Scientific Methodology; Scientific Research; Hands on Science; Instructional Effectiveness; Skill Development + Suchen Sie Ihr Suchwort? Biotechnologie; Molekularbiologie; Humangenetik; Contagious disease; Contagious diseases; Communicable disease; Infektionskrankheit; Unterrichtserfolg; Kompetenzentwicklung; Qualifikationsentwicklung
Abstract	Homologous recombination, a central concept in biology, is defined as the exchange of DNA strands between two similar or identical nucleotide sequences. Unfortunately, undergraduate students majoring in biotechnology often experience difficulties in understanding the molecular basis of homologous recombination. In this study, we developed and implemented a 12-week laboratory course for biotechnology undergraduates in which gene targeting in "Streptococcus suis" was used to facilitate their understanding of the basic concept and process of homologous recombination. Students worked in teams of two to select a gene of interest to create a knockout mutant using methods that relied on homologous recombination. By integrating abstract knowledge and practice in the process of scientific research, students gained hands-on experience in molecular biology techniques while learning about the principle and process of homologous recombination. The learning outcomes and survey-based assessment demonstrated that students substantially enhanced their understanding of how homologous recombination could be used to study gene function. Overall, the course was very effective for helping biotechnology undergraduates learn the theory and application of homologous recombination, while also yielding positive effects in developing confidence and scientific skills for future work in research. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01