Student-Designed Enzyme-Linked Metabolite Assay Kits

Autor/inn/en	Hancock, D.; Johnston, J.; Dimauro, J.; Denyer, G.
Titel	Student-Designed Enzyme-Linked Metabolite Assay Kits
Quelle	In: Biochemistry and Molecular Biology Education, 32 (2004) 5, S.326-330 (5 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.2004.494032050384
Schlagwörter	Leitfaden; Unterricht; Lehrer; Biochemistry; Molecular Biology; Teaching Methods; Metabolism; Student Developed Materials; Science Instruction; Peer Evaluation; Problem Solving; Design; Measurement Techniques + Suchen Sie Ihr Suchwort? Lesson concept; Instruction; Unterrichtsentwurf; Unterrichtsprozess; Teacher; Teachers; Lehrerin; Lehrende; Biochemie; Molekularbiologie; Teaching method; Lehrmethode; Unterrichtsmethode; Stoffwechsel; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Problemlösen; Messtechnik
Abstract	The extensive use of commercial kits in molecular biology and biochemistry has prompted us to design a series of practical sessions to help students become familiar with the uses and limitations of pre-packaged assay systems. To facilitate an understanding of these assay systems and to promote reflection on their appropriate use, students manufacture their own kit for one of four enzyme-linked metabolite assays. To do this they must investigate the role of each of the components in the assay, optimize the conditions where possible, check for cross reactivity, and then work this assay up for a few related "real" samples. Students make up all the buffers, the standard solution, instructions, and even the packaging. The kit is checked for accuracy by the producers, "marketed" to other students, and evaluated by their peers. By going through this process, students learn the benefits and pitfalls of commercial kits as well as reinforcing the basic principles of metabolite measurement and gaining experience with assay design, troubleshooting, and problem solving. (Author).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10