Varying Iron Release from Transferrin and Lactoferrin Proteins. A Laboratory Experiment

Autor/inn/en	Carmona, Fernando; González, Ana; Sánchez, Manu; Gálvez, Natividad; Cuesta, Rafael; Capdevila, Mercè; Dominguez-Vera, Jose M.
Titel	Varying Iron Release from Transferrin and Lactoferrin Proteins. A Laboratory Experiment
Quelle	In: Biochemistry and Molecular Biology Education, 45 (2017) 6, S.521-527 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Capdevila, Mercè) ORCID (Dominguez-Vera, Jose M.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21075
Schlagwörter	Laboratory Experiments; Metabolism; Undergraduate Students; Chemistry; Biochemistry; Science Instruction + Suchen Sie Ihr Suchwort? Laboratory work; Laborarbeit; Stoffwechsel; Chemie; Biochemie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht
Abstract	Iron metabolism is an important subject of study for undergraduate students of chemistry and biochemistry. Relevant laboratory exercises are scarce in the literature but would be very helpful in assisting students grasp key concepts. The experiment described here deals with different iron release mechanisms of two protagonists in iron metabolism: serum transferrin (Tf) and lactoferrin (Lf). Despite having very similar structures and iron-binding sites, Tf releases practically all its iron at pH 5.5 while Lf requires a significantly lower pH of 3. This difference in behavior is directly related to their respective biological functions as Tf blood-borne iron into the cell, while Lf competes with pathogens to sequester iron in biological fluids at more acidic pHs. During this experiment, the students will carry out iron loading and unloading on both human Lf and Tf and monitor the iron release at different pHs using UV-Vis spectroscopy. With this simple approach, the students will discover the different patterns of iron release of Tf and Lf and how this variance in behavior relates to their biological functions. Furthermore, this laboratory practice can be expanded to allow students to investigate a variety of iron proteins. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01