Nature's Cholesterol-Lowering Drug: Isolation and Structure Elucidation of Lovastatin from Red Yeast Rice-Containing Dietary Supplements

Autor/inn/en	Nazri, Maisarah Mohd; Samat, Farah D.; Kavanagh, Pierce V.; Walsh, John J.
Titel	Nature's Cholesterol-Lowering Drug: Isolation and Structure Elucidation of Lovastatin from Red Yeast Rice-Containing Dietary Supplements
Quelle	In: Journal of Chemical Education, 89 (2012) 1, S.138-140 (3 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/ed1004195
Schlagwörter	Dietetics; Organic Chemistry; Food; Science Instruction; Scientific Principles; College Science; Drug Therapy; Undergraduate Study; Science Laboratories; Spectroscopy; Pharmacy; Science Experiments + Suchen Sie Ihr Suchwort? Ernährungslehre; Organische Chemie; Lebensmittel; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Grundstudium; Spektroskopie; Apotheke
Abstract	Red yeast rice, produced by fermenting the fungus, "Monascus purpureus", on rice ("Oryza sativa" L. gramineae), is commonly used as a dietary supplement. It contains lovastatin, a member of the statin family of compounds, and is licensed for use as a cholesterol-lowering agent. This experiment involves the isolation and structure elucidation of lovastatin from red yeast rice-containing dietary supplements. Isolation of the neutral constituents in red yeast rice can be performed using either ether or ethyl acetate as extracting solvent, and flash column chromatography combined with thin-layer chromatography is used to isolate lovastatin in pure form from the extract. During the course of the experiment, the students isolate lovastatin with an average recovery of 0.31%. IR, MS, and detailed NMR spectroscopic analyses confirm the structure of lovastatin. The experiment can be completed over two, 3-h laboratory sessions and is suitable for students at the third- or fourth-year undergraduate level. Overall the experiment acquaints the student with the techniques required to isolate and complete structure elucidation studies on natural products. This experiment also highlights that dietary supplements may contain substances with potent pharmacological actions that are not expected from a cursory inspection of the labeled products. (Contains 1 figure.) (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10