Colorimetric Quantification and in Situ Detection of Collagen

Autor/inn/en	Esteban, Francisco J.; del Moral, Maria L.; Sanchez-Lopez, Ana M.; Blanco, Santos; Jimenez, Ana; Hernandez, Raquel; Pedrosa, Juan A.; Peinado, Maria A.
Titel	Colorimetric Quantification and in Situ Detection of Collagen
Quelle	In: Journal of Biological Education, 39 (2005) 4, S.183-186 (4 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9266
Schlagwörter	Undergraduate Students; Science Laboratories; Science Instruction; Correlation; Biochemistry; Anatomy; Diseases; Clinical Diagnosis; Teaching Methods + Suchen Sie Ihr Suchwort? Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Korrelation; Biochemie; Anatomie; Disease; Krankheit; Teaching method; Lehrmethode; Unterrichtsmethode
Abstract	A simple multidisciplinary and inexpensive laboratory exercise is proposed, in which the undergraduate student may correlate biochemical and anatomical findings. The entire practical session can be completed in one 2.5-3 hour laboratory period, and consists of the quantification of collagen and total protein content from tissue sections--without previous homogenisation--by a colorimetric assay, and a staining method for in situ collagen detection carried out on the same tissue samples using the same chemicals. Because collagen accumulation is a common characteristic of hepatic diseases such as fibrosis and cirrhosis, the experiment may also serve as the basis for discussing the importance of collagen detection and quantification in estimating the degree of fibrosis from subjects with liver disease. (Contains 2 figures and 4 tables.) (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10