Different Phases of Long-Term Memory Require Distinct Temporal Patterns of PKA Activity after Single-Trial Classical Conditioning

Autor/inn/en	Michel, Maximilian; Kemenes, Ildiko; Muller, Uli; Kemenes, Gyorgy
Titel	Different Phases of Long-Term Memory Require Distinct Temporal Patterns of PKA Activity after Single-Trial Classical Conditioning
Quelle	In: Learning & Memory, 15 (2008) 9, S.694-702 (9 Seiten) PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1072-0502
DOI	10.1101/lm.1088408
Schlagwörter	Classical Conditioning; Long Term Memory; Anatomy; Brain Hemisphere Functions; Food; Rewards; Animals; Molecular Structure; Measurement; Inhibition; Diagnostic Tests; Cytology + Suchen Sie Ihr Suchwort? Langzeitgedächtnis; Anatomie; Lebensmittel; Reward; Belohnung; Animal; Tier; Tiere; Messverfahren; Hemmung; Diagnostic test; Diagnostischer Test; Zytologie
Abstract	The cAMP-dependent protein kinase (PKA) is known to play a critical role in both transcription-independent short-term or intermediate-term memory and transcription-dependent long-term memory (LTM). Although distinct phases of LTM already have been demonstrated in some systems, it is not known whether these phases require distinct temporal patterns of learning-induced PKA activation. This question was addressed in a robust form of associative LTM that emerges within a matter of hours after single-trial food-reward classical conditioning in the pond snail "Lymnaea stagnalis". After establishing the molecular and functional identity of the PKA catalytic subunit in the "Lymnaea" nervous system, we used a combination of PKA activity measurement and inhibition techniques to investigate its role in LTM in intact animals. PKA activity in ganglia involved in single-trial learning showed a short latency but prolonged increase after classical conditioning. However, while increased PKA activity immediately after training (0-10 min) was essential for an early phase of LTM (6 h), the late phase of LTM (24 h) required a prolonged increase in PKA activity. These observations indicate mechanistically different roles for PKA in recent and more remote phases of LTM, which may underpin different cellular and molecular mechanisms required for these phases. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10