Critical Appraisal Tools Used in Systematic Reviews of in Vitro Cell Culture Studies: A Methodological Study

Autor/inn/en	Paiva Barbosa, Victor; Bastos Silveira, Bruna; Amorim dos Santos, Juliana; Monteiro, Mylene Martins; Coletta, Ricardo D.; De Luca Canto, Graziela; Stefani, Cristine Miron; Guerra, Eliete Neves Silva
Titel	Critical Appraisal Tools Used in Systematic Reviews of in Vitro Cell Culture Studies: A Methodological Study
Quelle	In: Research Synthesis Methods, 14 (2023) 6, S.776-793 (18 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Paiva Barbosa, Victor) ORCID (Bastos Silveira, Bruna) ORCID (Amorim dos Santos, Juliana) ORCID (Monteiro, Mylene Martins) ORCID (Coletta, Ricardo D.) ORCID (De Luca Canto, Graziela) ORCID (Stefani, Cristine Miron) ORCID (Guerra, Eliete Neves Silva)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1759-2879
DOI	10.1002/jrsm.1657
Schlagwörter	Cytology; Research; Research Methodology; Correlation; Data Collection; Literature Reviews; Measurement Techniques + Suchen Sie Ihr Suchwort? Zytologie; Forschung; Research method; Forschungsmethode; Korrelation; Data capture; Datensammlung; Messtechnik
Abstract	Systematic reviews (SRs) of preclinical studies are marked with poor methodological quality. In vitro studies lack assessment tools to improve the quality of preclinical research. This methodological study aimed to identify, collect, and analyze SRs based on cell culture studies to highlight the current appraisal tools utilized to support the development of a validated critical appraisal tool for cell culture in vitro research. SRs, scoping reviews, and meta-analyses that included cell culture studies and used any type of critical appraisal tool were included. Electronic search, study selection, data collection and methodological quality (MQ) assessment tool were realized. Further, statistical analyses regarding possible associations and correlations between MQ and collected data were performed. After the screening process, 82 studies remained for subsequent analysis. A total of 32 different appraisal tools were identified. Approximately 60% of studies adopted pre-structured tools not designed for cell culture studies. The most frequent instruments were SYRCLE (n = 14), OHAT (n = 9), Cochrane Collaboration's tool (n = 7), GRADE (n = 6), CONSORT (n = 5), and ToxRTool (n = 5). The studies were divided into subgroups to perform statistical analyses. A significant association (OR = 5.00, 95% CI = 1.54-16.20, p = 0.008) was found between low MQ and chronic degenerative disorders as topic of SR. Several challenges in collecting information from the included studies led to some modifications related to the previously registered protocol. These results may serve as a basis for further development of a critical appraisal tool for cell culture studies capable of capturing all the essential factors related to preclinical research, therefore enhancing the practice of evidence-based. (As Provided).
Anmerkungen	Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01