Novel, Simple and Inexpensive Programmed Lab Experiments to Evaluate the Biocontrol Activity of Rhizobacteria on Fungal Phytopathogens

Autor/inn/en	Foresto, Emiliano; Nievas, Fiorela; Giordano, Walter; Bogino, Pablo
Titel	Novel, Simple and Inexpensive Programmed Lab Experiments to Evaluate the Biocontrol Activity of Rhizobacteria on Fungal Phytopathogens
Quelle	In: Journal of Biological Education, 56 (2022) 5, S.581-597 (17 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Foresto, Emiliano) ORCID (Nievas, Fiorela) ORCID (Giordano, Walter) ORCID (Bogino, Pablo)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9266
DOI	10.1080/00219266.2020.1858934
Schlagwörter	Agronomy; Microbiology; Undergraduate Students; Experiments; Biological Sciences; Laboratory Experiments; Soil Science; Plants (Botany); Foreign Countries; Argentina + Suchen Sie Ihr Suchwort? Agarwissenschaft; Mikrobiologie; Erprobung; Abwasserbiologie; Laboratory work; Laborarbeit; Bodenkunde; Pflanze; Ausland; Argentinien
Abstract	Despite the growing importance of agricultural microbiology at the applied, productive and ecological levels, undergraduate degrees in Agronomy generally do not feature much microbiological experimentation. To address this deficiency, we have designed an experimental programme that evaluates the potential of plant growth-promoting rhizobacteria (PGPR) for the biocontrol of fungal pathogens. The approach involves an experimental laboratory class in which Agronomy students evaluate the ability of a rhizospheric "Burkholderia" sp. strain to inhibit the development of "Sclerotium rolfsii" (the causal agent of 'white wilt' disease in peanut plants). The assays cover three aspects: biochemical mechanisms associated to biocontrol properties in the bacterium, bacterium-fungus interaction (plate inhibition assays), and plant-bacterium-fungus interaction (disease control assays). Students verify the bacterial strain's ability to produce fungi-degrading enzymes, inhibit fungal growth, and protect the plant by inhibiting disease development. The assays serve as an integrative basis on which to discuss relationships between plants, bacteria, and fungi in connection with a disease which affects important regional crops. This set of experiments should help students to develop different skills which will be key in their future professional career, such as handling and designing experimental assays; comparing, discussing and solving practical problems; and drawing conclusions from their observations and the results obtained. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01