Quick and Cheap Colorimetric Quantification of Proteins Using 96-Well-Plate Images

Autor/inn/en	Filgueiras, Matheus Fernandes; Borges, Endler Marcel
Titel	Quick and Cheap Colorimetric Quantification of Proteins Using 96-Well-Plate Images
Quelle	In: Journal of Chemical Education, 99 (2022) 4, S.1778-1787 (10 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Borges, Endler Marcel)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.1c00756
Schlagwörter	Chemistry; Color; Visual Aids; Science Instruction; Food; College Science; Instructional Effectiveness + Suchen Sie Ihr Suchwort? Chemie; Colour; Farbbezeichnung; Farbe; Anschauungsmaterial; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Lebensmittel; Unterrichtserfolg
Abstract	Students quantified the protein content in beer, milk, powdered milk, and whey protein using the Bradford assay. The assay was carried out using absorbance measured at 595 nm (standard method) and 96-well-plate images (proposed method). They built analytical curves using bovine serum albumin (BSA) and casein and determined that protein type affected the Bradford assay. They also determined figures of merit such as the limit of detection (LOD), the limit of quantification (LOQ), linear working range, sensitivity, precision (standard deviation, F-test), and accuracy (percent error, t test). In addition, an interference test was carried out using nitrogen-rich compounds, organic bases, and amino acids, and it was shown that the Bradford assay was not affected by these compounds. Student learning outcomes were assessed by a final test using Microsoft Forms. As a learning model, students can determine the protein content in various food samples using just sample dilution, without the need for intricate sample preparation procedures. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01