Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper ("Pipernigrum" L.) Waste: An Undergraduate Chemistry Experiment

Autor/inn/en	Lucas, Danielle R.; Bressiani, Thaiany S. C.; Gomes, Anne C. C.; Hayashide, Ingrid M.; Simas, Naomi K.; Lelis, Maria F. F.; Kuster, Ricardo M.; Moura, Paulo R. G.
Titel	Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper ("Pipernigrum" L.) Waste: An Undergraduate Chemistry Experiment
Quelle	In: Journal of Chemical Education, 98 (2021) 4, S.1397-1403 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Lucas, Danielle R.) ORCID (Bressiani, Thaiany S. C.) ORCID (Lelis, Maria F. F.) ORCID (Moura, Paulo R. G.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.0c01106
Schlagwörter	Chemistry; Undergraduate Students; Science Experiments; Laboratory Experiments; Sanitation; Spectroscopy; Scientific Concepts + Suchen Sie Ihr Suchwort? Chemie; Laboratory work; Laborarbeit; Betriebshygiene; Spektroskopie
Abstract	A laboratory experiment was developed to introduce undergraduate chemistry students to the chemical characterization of agroindustrial waste extracts by gas chromatography-mass spectrometry (GC--MS). The students prepared hexane and dichloromethane extracts from black pepper ("Piper nigrum" L.) waste. GC--MS analysis revealed the possible presence of sesquiterpenes and piperine, compounds commonly found in "P. nigrum" fruits. During postlab discussion, the students were encouraged to search for information about the biological properties of the putatively identified compounds. Most students found that piperine shows larvicidal activity. Thus, an experiment was conducted by a postgraduate research group to investigate the larvicidal potential of "P. nigrum" waste extracts. From the results, the students concluded that the hexane extract had higher larvicidal activity than the dichloromethane extract. We also provided students with the opportunity to identify piperine using (+)-APCI FT-ICR MS spectra. The proposed experiment helped students to learn and practice GC--MS and stimulated them to search the scientific literature for solutions to environmental problems. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01