A Practical Approach for the Removal of Cationic Surfactants Using Planktonic and Immobilized Bacterial Cells

Autor/inn/en	Bergero, María F.; Boeris, Paola S.; Liffourrena, Andrés S.; Heredia, Romina M.; Lucchesi, Gloria I.
Titel	A Practical Approach for the Removal of Cationic Surfactants Using Planktonic and Immobilized Bacterial Cells
Quelle	In: Journal of Biological Education, 54 (2020) 5, S.561-567 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9266
DOI	10.1080/00219266.2019.1620316
Schlagwörter	Laboratory Procedures; College Science; Science Instruction; Undergraduate Students; Pollution; Microbiology; Science Laboratories; Cytology + Suchen Sie Ihr Suchwort? Laboruntersuchung; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Schadstoffbelastung; Mikrobiologie; Zytologie
Abstract	Among the efforts directed at remediating contaminated sites, the use of immobilized microorganisms is an appealing alternative because it offers several advantages over the use of free cells, including protecting cells from the toxic effects of hazardous compounds and increasing their survival and metabolic activity. "Pseudomonas putida" is an ubiquitous bacterium which, either as free cells (i.e. in its planktonic state) or immobilized in Ca-alginate beads, is able to effectively degrade pollutants such as cationic surfactants. In this paper, we propose a simple and fast technique to immobilize "P. putida" cells by entrapment in Ca-alginate matrix, and the use of this system and the free cells to estimate the removal of the surfactant Br-tetradecyltrimethylammonium (TTAB). Measurement of residual TTAB with a simple colorimetric method shows that the compound can be removed both by planktonic and immobilized cells. Taking into account that the TTAB-removal rate is higher with immobilized cells than with free cells, the laboratory practical class aims at demonstrating one of the advantages of using immobilized cells in remediation processes. The class is addressed to undergraduate students of biological sciences, engineering and biotechnology, and it could also be applied to evaluate the removal of other pollutants with immobilized microorganisms. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01