Pathway-Based Meta-Analysis for Partially Paired Transcriptomics Analysis

Autor/inn/en	Fung, Wing Tung; Wu, Joseph T.; Chan, Wai Man Mandy; Chan, Henry H.; Pang, Herbert
Titel	Pathway-Based Meta-Analysis for Partially Paired Transcriptomics Analysis
Quelle	In: Research Synthesis Methods, 11 (2020) 1, S.123-133 (11 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Pang, Herbert)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1759-2879
DOI	10.1002/jrsm.1381
Schlagwörter	Meta Analysis; Genetics; Biomedicine; Medical Research; Diseases; Profiles; Effect Size; Cytology; Human Body; Guidelines + Suchen Sie Ihr Suchwort? Meta-analysis; Metaanalyse; Humangenetik; Biomedizin; Disease; Krankheit; Charakterisierung; Profilanalyse; Zytologie; Menschlicher Körper; Richtlinien
Abstract	Pathway-based differential expression analysis allows the incorporation of biological domain knowledge into transcriptomics analysis to enhance our understanding of disease mechanisms. To integrate information among multiple studies at the pathway level, pathway-based meta-analysis can be performed. Paired or partially paired samples are common in biomedical research. However, there are currently no existing pathway-based meta-analysis methods appropriate for paired or partially paired study designs. In this study, we developed a pathway-based meta-analysis approach for paired or partially paired samples. Meta-analysis on the transcriptomics profiles were conducted using p-value-based, rank-based, and effect size-based algorithms. The application of our approach was demonstrated using partially paired data from psoriasis transcriptomics studies. Upon combining six transcriptomics studies, genes related to the cell cycle and DNA replication pathways are found to be highly perturbed in psoriatic lesional skin samples. Results were validated externally with independent RNA-Seq data. Comparison with existing pathway meta-analysis methods revealed consistent results, with our method showing higher detection power. This study demonstrated the utility of our newly developed pathway-based meta-analysis that allows the incorporation of partially paired or paired samples. The proposed framework can be applied to omics data including but not limited to transcriptomics data. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01