Using NMR Spectroscopy to Measure Protein Binding Capacity on Gold Nanoparticles

Autor/inn/en	Perera, Y. Randika; South, Taylor M.; Hughes, Alex C.; Parkhurst, Ashlyn N.; Williams, Olivia C.; Davidson, Mackenzie B.; Wilks, Chloe A.; Mlsna, Debra A.; Fitzkee, Nicholas C.
Titel	Using NMR Spectroscopy to Measure Protein Binding Capacity on Gold Nanoparticles
Quelle	In: Journal of Chemical Education, 97 (2020) 3, S.820-824 (5 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Mlsna, Debra A.) ORCID (Fitzkee, Nicholas C.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
Schlagwörter	Teaching Methods; Spectroscopy; Science Instruction; Laboratory Experiments; Drug Therapy; Diagnostic Tests; Biochemistry; Electronic Equipment; Measurement; Cytology; Scientific Concepts; Molecular Structure + Suchen Sie Ihr Suchwort? Teaching method; Lehrmethode; Unterrichtsmethode; Spektroskopie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Laboratory work; Laborarbeit; Diagnostic test; Diagnostischer Test; Biochemie; Elektronisches Gerät; Messverfahren; Zytologie
Abstract	A simple one-dimensional [superscript 1]H NMR experiment that quantifies protein bound to gold nanoparticles has been developed for upper-division biochemistry and physical chemistry students. This laboratory experiment teaches the basics of NMR techniques, which is a highly effective tool in protein studies and supports students to understand the concepts of NMR spectroscopy and nanoparticle-protein interactions. Understanding the interactions of gold nanoparticles (AuNPs) with biological macromolecules is becoming increasingly important as interest in the clinical use of nanoparticles has been on the rise. Applications in drug delivery, biosensing, diagnostics, and enhanced imaging are all tangible possibilities with a better understanding of AuNP-protein interactions. The ability to use AuNPs as biosensors for drug delivery methods in cellular uptake is dependent on the amount of protein that is able to bind to the surface of the nanoparticle. This laboratory experiment solidifies concepts such as quantitative NMR spectroscopy while reinforcing precision laboratory titrations. Students learn how [superscript 1]H proton NMR spectra can be used to measure free protein in solution and protein bound to AuNPs. A simple formula is used to determine the binding capacity of the nanoparticle. This analysis helps students to understand the impact of nanoparticle-protein interactions, and it allows them to conceptualize macromolecular binding using NMR spectroscopy. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01