Inhibitory Learning of Phototaxis by Honeybees in a Passive-Avoidance Task

Autor/inn/en	Marchal, Paul; Villar, Maria Eugenia; Geng, Haiyang; Arrufat, Patrick; Combe, Maud; Viola, Haydée; Massou, Isabelle; Giurfa, Martin
Titel	Inhibitory Learning of Phototaxis by Honeybees in a Passive-Avoidance Task
Quelle	In: Learning & Memory, 26 (2019) 10, S.412-423 (12 Seiten) PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1072-0502
DOI	10.1101/lm.050120.119
Schlagwörter	Inhibition; Learning Processes; Memory; Molecular Structure; Entomology; Task Analysis; Light; Operant Conditioning; Brain; Genetics; Biochemistry; Cytology; Comparative Analysis + Suchen Sie Ihr Suchwort? Hemmung; Learning process; Lernprozess; Gedächtnis; Entomologie; Aufgabenanalyse; Licht; Operante Konditionierung; Gehirn; Humangenetik; Biochemie; Zytologie
Abstract	Honeybees are a standard model for the study of appetitive learning and memory. Yet, fewer attempts have been performed to characterize aversive learning and memory in this insect and uncover its molecular underpinnings. Here, we took advantage of the positive phototactic behavior of bees kept away from the hive in a dark environment and established a passive-avoidance task in which they had to suppress positive phototaxis. Bees placed in a two-compartment box learned to inhibit spontaneous attraction to a compartment illuminated with blue light by associating and entering into that chamber with shock delivery. Inhibitory learning resulted in an avoidance memory that could be retrieved 24 h after training and that was specific to the punished blue light. The memory was mainly operant but involved a Pavlovian component linking the blue light and the shock. Coupling conditioning with transcriptional analyses in key areas of the brain showed that inhibitory learning of phototaxis leads to an upregulation of the dopaminergic receptor gene "Amdop1" in the calyces of the mushroom bodies, consistently with the role of dopamine signaling in different forms of aversive learning in insects. Our results thus introduce new perspectives for uncovering further cellular and molecular underpinnings of aversive learning and memory in bees. Overall, they represent an important step toward comparative learning studies between the appetitive and the aversive frameworks. (As Provided).
Anmerkungen	Cold Spring Harbor Laboratory Press. 500 Sunnyside Boulevard, Woodbury, NY 11797-2924. Tel: 800-843-4388; Tel: 516-367-8800; Fax: 516-422-4097; e-mail: cshpres@cshl.edu; Web site: http://learnmem.cshlp.org
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01