Comparing Free Radicals in Sunscreen-Treated Pig Skin by Using Electron Paramagnetic Resonance Spectroscopy

Autor/inn/en	Quiñones, Rosalynn; Kolling, Derrick R. J.; Shoup, Deben; Smythers, Amanda L.; Nickel, Sarah; Westfall,Tamara D.; Epperly, Courtney; Coplin, Miranda
Titel	Comparing Free Radicals in Sunscreen-Treated Pig Skin by Using Electron Paramagnetic Resonance Spectroscopy
Quelle	In: Journal of Chemical Education, 96 (2019) 9, (8 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Quiñones, Rosalynn)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
Schlagwörter	Spectroscopy; Animals; Cancer; Radiation; Human Body; Science Experiments; Undergraduate Students; Data Interpretation; Relevance (Education); Organic Chemistry; Merchandise Information + Suchen Sie Ihr Suchwort? Spektroskopie; Animal; Tier; Tiere; Carcinoma; Karzinom; Krebs (med); Krebserkrankung; Strahlung; Menschlicher Körper; Data evaluation; Datenauswertung; Relevance; Relevanz; Organische Chemie
Abstract	Melanoma, a type of cancer that develops in melanocytes, is usually caused by direct exposure of skin to ultraviolet (UV) radiation resulting in cellular damage. In this study, a procedure to determine the effects of various commercial sunscreens with SPF values ranging from 15 to 100 was developed using pig skin to mimic human skin. These sunscreens contain inorganic filters, such as zinc oxide and titanium dioxide; active organic ingredients, such as octocrylene and oxybenzone; or both. As a model for human skin, pig skin was analyzed before and after UV exposure, and the presence of free radicals was measured using electron paramagnetic resonance (EPR) spectroscopy. Using this method, students were able to quantify radical formation following irradiation and use this as a basis to compare the efficacies of sunscreens against UVA. This experiment allowed undergraduate students to characterize a complex chemical process (light-induced radical formation) and relate it to something they experience every day (sun damage). Interestingly, students found higher levels of postillumination radical formation in sunscreen-treated samples, perhaps indicating sunscreen-induced stabilization of these species. Student outcomes included learning how to collect and interpret EPR data, statistical analysis of these data, and the preparation of reproducible biological samples. Students also consulted literature sources to properly display their measurements. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01