Monoclonal Antibody Production: A Project-Based Laboratory Program for Final Year Biotechnology Undergraduate Students

Autor/in	Lindsay, Andrew J.
Titel	Monoclonal Antibody Production: A Project-Based Laboratory Program for Final Year Biotechnology Undergraduate Students
Quelle	In: Journal of Chemical Education, 96 (2019) 9, S.2036-2041 (6 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Lindsay, Andrew J.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
Schlagwörter	Biotechnology; Undergraduate Students; Student Projects; Active Learning; Laboratory Experiments; Science Instruction; Biochemistry; Cytology; Quality Control; Information Literacy; Theses; Teaching Methods; Spreadsheets + Suchen Sie Ihr Suchwort? Biotechnologie; Schulprojekt; Aktives Lernen; Laboratory work; Laborarbeit; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Biochemie; Zytologie; Qualitätskontrolle; Informationskompetenz; These; Teaching method; Lehrmethode; Unterrichtsmethode; Spread sheet; Spredsheets; Spreadsheet; Tabellenkalkulation
Abstract	A semester-long, project-based series of laboratory exercises is described that provides practical experience of the upstream and downstream processes involved in monoclonal antibody production. This 6 week project is aimed at final year undergraduate students undertaking primary degrees in biotechnology, biochemistry, or related disciplines. Students develop subject matter knowledge of biologics production and practical skills related to cell culture, column chromatography, quality control, and information literacy. Students are assigned a vial containing a hybridoma cell line stored in liquid nitrogen. They thaw the vial and culture the hybridoma over the course of 4 weeks. They perform daily counts of live/dead cells and quantify glucose levels in the media. During the 4 weeks they will expand their cells from a single 60 mm dish into two large T-175 flasks, one for antibody production and one for generating stocks. They harvest the medium from the production flask and purify the antibody via Protein G affinity chromatography on an FPLC instrument. The purity of the antibody is tested by SDS-PAGE, and the concentration is determined on a Nanodrop instrument. Students present their results in the form of a project thesis, a product datasheet, and a public presentation. Learning was assessed relative to six learning objectives using a student survey administered at the beginning (presurvey) and end (postsurvey). The students who have undertaken this module demonstrated substantial learning gains. This laboratory project is highly adaptable with scope to adapt it for longer or shorter study periods. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01