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Autor/inn/enStankus, Brian; White, Rosemary; Abrams, Binyomin
TitelEffective and Inexpensive HPLC Analogue for First-Year Students: Buret Chromatography of Food Dyes in Drinks
QuelleIn: Journal of Chemical Education, 96 (2019) 4, S.739-744 (6 Seiten)Infoseite zur Zeitschrift
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ZusatzinformationORCID (Abrams, Binyomin)
Spracheenglisch
Dokumenttypgedruckt; online; Zeitschriftenaufsatz
ISSN0021-9584
DOI10.1021/acs.jchemed.8b00665
SchlagwörterChemistry; Science Instruction; College Science; Undergraduate Study; Science Laboratories; Secondary School Science; High Schools; Laboratory Equipment; Food; Spectroscopy
AbstractChromatography is a ubiquitous technique in analytical chemistry, and high-performance liquid chromatography (HPLC) is the industry standard for quantitation of components in solution mixtures. However, demonstrating the principles of liquid chromatography to undergraduates via HPLC-based experiments often proves ineffective because of the fully automated nature of modern HPLC units. This paper details a novel laboratory exercise that is accessible to students in first-year chemistry and high school and helps to demonstrate and demystify the principles involved in HPLC. Using only common and inexpensive laboratory equipment found in most general-chemistry laboratories (burets and benchtop spectrophotometers), students are able to replicate results that are equivalent to those obtained on research-grade HPLC instruments, while gaining valuable insight into chromatography principles. (As Provided).
AnmerkungenDivision of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst vonERIC (Education Resources Information Center), Washington, DC
Update2020/1/01
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