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Autor/inn/en | Stankus, Brian; White, Rosemary; Abrams, Binyomin |
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Titel | Effective and Inexpensive HPLC Analogue for First-Year Students: Buret Chromatography of Food Dyes in Drinks |
Quelle | In: Journal of Chemical Education, 96 (2019) 4, S.739-744 (6 Seiten)Infoseite zur Zeitschrift
PDF als Volltext |
Zusatzinformation | ORCID (Abrams, Binyomin) |
Sprache | englisch |
Dokumenttyp | gedruckt; online; Zeitschriftenaufsatz |
ISSN | 0021-9584 |
DOI | 10.1021/acs.jchemed.8b00665 |
Schlagwörter | Chemistry; Science Instruction; College Science; Undergraduate Study; Science Laboratories; Secondary School Science; High Schools; Laboratory Equipment; Food; Spectroscopy |
Abstract | Chromatography is a ubiquitous technique in analytical chemistry, and high-performance liquid chromatography (HPLC) is the industry standard for quantitation of components in solution mixtures. However, demonstrating the principles of liquid chromatography to undergraduates via HPLC-based experiments often proves ineffective because of the fully automated nature of modern HPLC units. This paper details a novel laboratory exercise that is accessible to students in first-year chemistry and high school and helps to demonstrate and demystify the principles involved in HPLC. Using only common and inexpensive laboratory equipment found in most general-chemistry laboratories (burets and benchtop spectrophotometers), students are able to replicate results that are equivalent to those obtained on research-grade HPLC instruments, while gaining valuable insight into chromatography principles. (As Provided). |
Anmerkungen | Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc |
Erfasst von | ERIC (Education Resources Information Center), Washington, DC |
Update | 2020/1/01 |