A Toolkit to Quantify Target Compounds in Thin-Layer-Chromatography Experiments

Autor/inn/en	Mac Fhionnlaoich, Niamh; Ibsen, Stuart; Serrano, Luis A.; Taylor, Alaric; Qi, Runzhang; Guldin, Stefan
Titel	A Toolkit to Quantify Target Compounds in Thin-Layer-Chromatography Experiments
Quelle	In: Journal of Chemical Education, 95 (2018) 12, S.2191-2196 (6 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Mac Fhionnlaoich, Niamh) ORCID (Serrano, Luis A.) ORCID (Taylor, Alaric) ORCID (Qi, Runzhang) ORCID (Guldin, Stefan)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.8b00144
Schlagwörter	Science Instruction; Chemistry; College Science; Undergraduate Study; Secondary School Science; High Schools; Scientific Principles; Educational Technology; Technology Uses in Education; Science Experiments; Telecommunications; Handheld Devices; Science Laboratories; Laboratory Experiments + Suchen Sie Ihr Suchwort? Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Chemie; Grundstudium; High school; Oberschule; Unterrichtsmedien; Technology enhanced learning; Technology aided learning; Technologieunterstütztes Lernen; Telekommunikationstechnik; Laboratory work; Laborarbeit
Abstract	Thin-layer chromatography (TLC) is one of the basic analytical procedures in chemistry and allows the demonstration of various chemical principles in an educational setting. An often-overlooked aspect of TLC is the capability to quantify isolated target compounds in an unknown sample. Here, we present a suitable route to implement quantitative analysis in a lesson plan. We provide both a stand-alone software and an online webapp that allow students to obtain quantitative information from a developed TLC plate and present two suitable experiments, namely, the absorbance-based quantification of the colorant Sudan IV and the fluorescence-based quantification of rhodamine 6G, a fluorophore widely used in biotechnology. Students conduct TLC experiments following established protocols, take pictures of their TLC plates with mobile phones, and subsequently quantify the different compounds in the separate bands they observe. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01