Determination of Xylitol in Sugar-Free Gum by GC-MS with Direct Aqueous Injection: A Laboratory Experiment for Chemistry Students

Autor/inn/en	Rajapaksha, Suranga M.; Samarasekara, Dulani; Brown, John Charles; Howard, Leslie; Gerken, Katherine; Archer, Todd; Lathan, Patty; Mlsna, Todd; Mlsna, Deb
Titel	Determination of Xylitol in Sugar-Free Gum by GC-MS with Direct Aqueous Injection: A Laboratory Experiment for Chemistry Students
Quelle	In: Journal of Chemical Education, 95 (2018) 11, S.2017-2022 (6 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Mlsna, Todd) ORCID (Mlsna, Deb)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.8b00424
Schlagwörter	Science Instruction; Science Laboratories; Laboratory Experiments; Science Experiments; Undergraduate Students; College Science; Spectroscopy; Organic Chemistry; Animals + Suchen Sie Ihr Suchwort? Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Laboratory work; Laborarbeit; Spektroskopie; Organische Chemie; Animal; Tier; Tiere
Abstract	Proficiency with instrumental analysis via GC-MS is an important skill for chemistry students. The application of analytical techniques and fundamental theoretical principles to real-world problems can be valuable learning exercises for undergraduates which can also improve their analytical thinking skills. Xylitol is generally considered safe for human consumption and is frequently used in sugar-free gum; however, it is extremely toxic to dogs. In this laboratory experiment, upper-level undergraduate chemistry students extract xylitol from both fresh and chewed gum sticks followed by direct aqueous injection GC-MS analysis. Students learn the proper steps and techniques required for sample extraction and preparation and GC--MS analysis, and they determine concentrations of xylitol present in gum samples. Identification and quantification of the chemical components in gum extract occurs via GC-MS analysis; however, other GC detectors could be used. Students also compare external and internal standard calibration methods for xylitol quantification. Upon quantification of xylitol in chewed and unchewed gum samples, students are able to calculate the level of hazard for dogs upon ingestion. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01