Colorimetric Measurements of Amylase Activity: Improved Accuracy and Efficiency with a Smartphone

Autor/inn/en	Dangkulwanich, Manchuta; Kongnithigarn, Kaness; Aurnoppakhun, Nattapat
Titel	Colorimetric Measurements of Amylase Activity: Improved Accuracy and Efficiency with a Smartphone
Quelle	In: Journal of Chemical Education, 95 (2018) 1, S.141-156 (5 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Dangkulwanich, Manchuta)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.7b00468
Schlagwörter	Telecommunications; Handheld Devices; Educational Technology; Technology Uses in Education; Spectroscopy; College Science; Chemistry; Undergraduate Study; Light; Science Laboratories; Laboratory Experiments; Science Experiments; Scientific Concepts; Hands on Science; Foreign Countries; Thailand + Suchen Sie Ihr Suchwort? Telekommunikationstechnik; Unterrichtsmedien; Technology enhanced learning; Technology aided learning; Technologieunterstütztes Lernen; Spektroskopie; Chemie; Grundstudium; Licht; Laboratory work; Laborarbeit; Ausland
Abstract	Routinely used in quantitative determination of various analytes, UV-vis spectroscopy is commonly taught in undergraduate chemistry laboratory courses. Because the technique measures the absorbance of light through the samples, losses from reflection and scattering by large molecules interfere with the measurement. To emphasize the importance of light scattering in UV-vis spectroscopy, a laboratory experiment is described that guides students to study the hydrolysis of starch by amylase as a function of temperature. The reducing sugar produced from the hydrolysis is determined by dinitrosalicylic colorimetric assay via two methods: UV-vis spectroscopy and a smartphone-based colorimetry, which is capable of quantifying the concentration of a chromogenic compound in an opaque sample. From this experiment, students can directly learn to measure the activity of amylase, use a smartphone to perform colorimetry, observe the effect of light scattering, and gain a better understanding of UV-vis spectroscopy. (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01