Biological Interaction of Molybdenocene Dichloride with Bovine Serum Albumin Using Fluorescence Spectroscopy

Autor/inn/en	Domínguez, Moralba; Cortes-Figueroa, Jose´ E.; Meléndez, Enrique
Titel	Biological Interaction of Molybdenocene Dichloride with Bovine Serum Albumin Using Fluorescence Spectroscopy
Quelle	In: Journal of Chemical Education, 95 (2018) 1, S.152- (6 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Meléndez, Enrique)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.7b00178
Schlagwörter	Chemistry; Science Instruction; College Science; Undergraduate Study; Molecular Structure; Science Laboratories; Hands on Science; Thermodynamics; Metallurgy; Spectroscopy; Science Experiments; Laboratory Experiments; Puerto Rico + Suchen Sie Ihr Suchwort? Chemie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Grundstudium; Thermodynamik; Hüttenwesen; Spektroskopie; Laboratory work; Laborarbeit
Abstract	Bioinorganic topics are ubiquitous in the inorganic chemistry curriculum; however, experiments to enhance understanding of related topics are scarce. In this proposed laboratory, upper undergraduate students assess the biological interaction of molybdenocene dichloride (Cp2MoCl2) with bovine serum albumin (BSA) by fluorescence spectroscopy. Specifically, learners study the quenching mechanism by performing a binding titration of a bovine serum albumin (BSA) solution with molybdenocene dichloride at physiological pH at three temperatures to determine the biomolecular quenching constant as defined in the Stern-Volmer equation. The temperature dependency of the quenching constant allows estimation of thermodynamic parameters which in turn permits an assessment of the nature of the intermolecular interactions involved. This educational activity promotes graph interpretation and integration of concepts such as metallocene-protein interaction, fluorescence quenching, Gibbs energy, entropy, and enthalpy, where students learn to propose a quenching mechanism and to assess the intermolecular forces that may be involved. The proposed experiment can be implemented in various educational settings such as inorganic chemistry, biochemistry, biophysical chemistry, and analytical chemistry. (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01