Different Cells Make Different Proteins: A Laboratory Exercise Illustrating Tissue-Specific Protein Expression in Animals

Autor/inn/en	Ibarguren, Izaskun; Villamarín, Antonio
Titel	Different Cells Make Different Proteins: A Laboratory Exercise Illustrating Tissue-Specific Protein Expression in Animals
Quelle	In: Journal of Biological Education, 51 (2017) 3, S.284-294 (11 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Villamarín, Antonio)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9266
DOI	10.1080/00219266.2016.1217908
Schlagwörter	Biology; Animals; Cytology; Genetics; Undergraduate Students; College Science; Science Laboratories; Scientific Concepts; Concept Formation; Science Process Skills; Veterinary Medical Education; Student Attitudes; Science Experiments; Laboratory Experiments + Suchen Sie Ihr Suchwort? Biologie; Animal; Tier; Tiere; Zytologie; Humangenetik; Concept learning; Begriffsbildung; Schülerverhalten; Laboratory work; Laborarbeit
Abstract	All the cells of higher organisms have the same DNA but not the same proteins. Each type of specialised cell that forms a tissue has its own pattern of gene expression and, consequently, it contains a particular set of proteins that determine its function. Here, we describe a laboratory exercise addressed to undergraduate students that aims to reinforce the following concept: what makes cells different is they make different proteins. The students performed a denaturing polyacrylamide gel electrophoresis (SDS-PAGE) to separate the proteins extracted from different animal tissues, and then obtained and compared the corresponding protein profiles. In addition, the students performed a Western blot analysis to detect the presence or absence of a tissue-specific protein in the different tissue extracts. This laboratory exercise allowed students to understand the basis of the cell differentiation better and also provided them the opportunity to learn a variety of analytical laboratory techniques. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01