A DNA Melting Exercise for a Large Laboratory Class

Autor/inn/en	Levine, Lauren A.; Junker, Matthew; Stark, Myranda; Greenleaf, Dustin
Titel	A DNA Melting Exercise for a Large Laboratory Class
Quelle	In: Journal of Chemical Education, 92 (2015) 11, S.1928-1931 (4 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.5b00049
Schlagwörter	Laboratory Experiments; Science Activities; Genetics; Large Group Instruction; Thermodynamics; Spectroscopy; Stoichiometry; Undergraduate Students; Biochemistry; Safety; Teaching Methods; Educational Practices + Suchen Sie Ihr Suchwort? Laboratory work; Laborarbeit; Humangenetik; Thermodynamik; Spektroskopie; Stöchiometrie; Biochemie; Sicherheit; Teaching method; Lehrmethode; Unterrichtsmethode; Bildungspraxis
Abstract	A simple and economical experimental setup is described that enables multiple individuals or groups within a laboratory class to measure the thermal melting of double stranded DNA simultaneously. The setup utilizes a basic spectrophotometer capable of measuring absorbance at 260 nm, UV plastic cuvettes, and a stirring hot plate. Students measure melting curves of short matched and mismatched DNA duplexes, and use quantitative analysis and first derivative curves to determine each duplex's transition melting temperature, T[subscript m]. Student data are highly reproducible among groups and from year to year, and give distinct transition melting temperatures for the different duplexes. The protocol could easily be adapted to investigate the effects of other parameters on DNA melting and stability, such as changes in base composition, chain length, and buffer ionic strength. (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01