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| Autor/Urheber | Coelho R; Marcos-Silva N; Mendes N; Pereira D; Brito C; Jacob F; Steentoft C; Mandel U; Clausen H; David L; Ricardo S |
|---|---|
| Institution | MDPI |
| Titel | Mucins and truncated O-glycans unveil phenotypic discrepancies between serous ovarian cancer cell lines and primary tumours. |
| Quelle | In: International Journal of Molecular Sciences, vol.19(7):2045; 1661-6596; doi:10.3390/ijms19072045(2018)
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| Sprache | englisch |
| Dokumenttyp | online; Zeitschriftenaufsatz |
| DOI | 10.3390/ijms19072045 |
| Schlagwörter | Animals; Biomarkers; Tumor; CA-125 Antigen; Cystadenocarcinoma; Serous; Female; Gene Expression Profiling; Glycosylation; Humans; Membrane Proteins; Mice; Nude; Molecular Chaperones; Mucin-1; Ovarian Neoplasms; Phenotype; Polysaccharides; Transplantation; Heterologous |
| Abstract | Optimal research results rely on the selection of cellular models capable of recapitulating the characteristics of primary tumours from which they originate. The expression of mucins (MUC16 and MUC1) and truncated O-glycans (Tn, STn and T) represents a characteristic footprint of serous ovarian carcinomas (SOCs). Therefore, selecting ovarian cancer (OVCA) cell lines that reflect this phenotype is crucial to explore the putative biological role of these biomarkers in the SOC setting. Here, we investigated a panel of OVCA cell lines commonly used as SOC models, and tested whether, when cultured in 2D and 3D conditions, these recapitulate the mucin and O-glycan expression profiles of SOCs. We further explored the role of truncating the O-glycosylation capacity in OVCAR3 cells through knockout of the COSMC chaperone, using in vitro and in vivo assays. We found that the majority of OVCA cell lines of serous origin do not share the mucin and truncated O-glycan footprint of SOCs, although 3D cultures showed a higher resemblance. We also found that genetic truncation of the O-glycosylation capacity of OVCAR3 cells did not enhance oncogenic features either in vitro or in vivo. This study underscores the importance of well-characterized cellular models to study specific features of ovarian cancer. ; Funding: IPATIMUP is an Associate Laboratory of the Portuguese Ministry of Science, Technology and Higher Education and partially supported by Fundação para a Ciência e a Tecnologia (FCT, I.P.). This research was funded by NORTE 2020 grant numbers NORTE-01-0145-FEDER-000029 and NORTE-01-0145-FEDER-000003. Ricardo Coelho acknowledges FCT, I.P. for financial support through a PhD fellowship number SFRH/BD/111885/2015. Acknowledgments: We acknowledge Tatiana Martins for all the support regarding 3D cultures and projects: KFS-3841-02-2016 from Krebsliga Schweiz, DNRF107 from the Danish National Research Foundation, UID/Multi/04462/2013 from iNOVA4Health (program financially supported by Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement). |
| Erfasst von | BASE - Bielefeld Academic Search Engine |
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