Ultraviolet-B radiation exposure lowers the antioxidant capacity in the Arabidopsis thaliana pdx1.3-1 mutant and leads to glucosinolate biosynthesis alteration in both wild type and mutant.

Autor/Urheber	Neugart, Susanne; Hideg, Éva; Czégény, Gyula; Schreiner, Monika; Strid, Åke
Institution	Örebro universitet, Institutionen för naturvetenskap och teknik; Division of Quality and Sensory of Plant Products, University of Göttingen, Göttingen, Germany; Department of Plant Biology, University of Pécs, Pécs, Hungary; Department of Plant Quality and Food Security, Leibniz Institute of Vegetable and Ornamental Crops, Großbeeren, Germany
Titel	Ultraviolet-B radiation exposure lowers the antioxidant capacity in the Arabidopsis thaliana pdx1.3-1 mutant and leads to glucosinolate biosynthesis alteration in both wild type and mutant.
Quelle	In: Photochemical and Photobiological Sciences, 1474-905X, 2020, 19:2, s. 217-228; doi:10.1039/C9PP00342H; PMID 31961357; ISI:000516590600006; Scopus 2-s2.0-85081943991(2020) PDF als Volltext kostenfreie Datei
Sprache	englisch
Dokumenttyp	online; Zeitschriftenaufsatz
DOI	10.1039/C9PP00342H
Schlagwörter	Botany; Botanik; Biochemistry and Molecular Biology; Biokemi och molekylärbiologi; Analytical Chemistry; Analytisk kemi
Abstract	Pyridoxine (vitamin B6) and its vitamers are used by living organisms both as enzymatic cofactors and as antioxidants. We used Arabidopsis pyridoxine biosynthesis mutant pdx1.3-1to study involvement of the PLP-synthase main polypeptide PDX1 in plant responses to ultraviolet radiation of two different qualities, one containing primarily UV-A (315-400 nm), the other containing both UV-A and UV-B (280-315 nm). The antioxidant capacity and the flavonoid and glucosinolate (GS) profiles were examined. As indicator of stress, Fv/Fmof photosystem II reaction centers was used. In pdx1.3-1, UV-A+B exposure led to a significant 5% decrease in Fv/Fmon the last day (day 15), indicating mild stress at this time point. Antioxidant capacity of Col-0 wildtype increased significantly (50-73%) after 1 and 3 days of UV-A+B. Instead, in pdx1.3-1, the antioxidant capacity significantly decreased by 44-52% over the same time period, proving the importance of a full complement of functional PDX1genes for detoxification of reactive oxygen species. There were no significant changes in flavonoid glycoside profile under any light condition. However, the GS profile was significantly altered, both with respect to Arabidopsis accession and exposure to UV. The difference in flavonoid and GS profiles reflect that the GS biosynthesis pathway contains at least one pyridoxine-dependent enzyme, whereas no such enzyme is used in flavonoid biosynthesis. Also, there was strong correlation between the antioxidant capacity and the content of some GS compounds. Our results show that vitamin B6vitamers, functioning both as antioxidants and co-factors, are of importance for physiological fitness of plants. ; Funding Agencies: Örebro University's Faculty for Business, Science and Technology Higher Education Institutional Excellence Programme of the Ministry of Human Capacities in Hungary 20765-3/2018/FEKUTSTRAT Research Area Plant Quality and Food Security at Leibniz-Institute of Vegetable and Ornamental Crops ; UV4quality
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