Confirming the 3D Solution Structure of a Short Double-Stranded DNA Sequence Using NMR Spectroscopy

Autor/inn/en	Ruhayel, Rasha A.; Berners-Price, Susan J.
Titel	Confirming the 3D Solution Structure of a Short Double-Stranded DNA Sequence Using NMR Spectroscopy
Quelle	In: Journal of Chemical Education, 87 (2010) 7, S.732-734 (3 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/ed100271h
Schlagwörter	Spectroscopy; College Science; Chemistry; Advanced Courses; Genetics; Cancer; Drug Therapy; Science Experiments; Molecular Structure; Models + Suchen Sie Ihr Suchwort? Spektroskopie; Chemie; Fortgeschrittenenunterricht; Humangenetik; Carcinoma; Karzinom; Krebs (med); Krebserkrankung; Analogiemodell
Abstract	2D [superscript 1]H NOESY NMR spectroscopy is routinely used to give information on the closeness of hydrogen atoms through space. This work is based on a 2D [superscript 1]H NOESY NMR spectrum of a 12 base-pair DNA duplex. This 6-h laboratory workshop aims to provide advanced-level chemistry students with a basic, yet solid, understanding of how the 3D structure of a double-stranded DNA sequence can be determined using 2D [superscript 1]H NOESY NMR spectroscopy and, hence, how structural perturbations induced by DNA binding agents such as platinum anticancer drugs are studied. Students are supplied with various sections of the 2D [superscript 1]H NOESY spectrum of the DNA sequence. Using these, as well as a molecular model as a visual aid and tool for measuring interproton distances, they are able to sequentially assign the structure of the DNA. (Contains 2 notes and 2 figures.) (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10