GABA[sub A] Receptor-Dependent Synchronization Leads to Ictogenesis in the Human Dysplastic Cortex

Autor/inn/en	D'Antuono, M.; Louvel, J.; Kohling, R.; Mattia, D.; Bernasconi, A.; Olivier, A.; Turak, B.; Devaux, A.; Pumain, R.; Avoli, M.
Titel	GABA[sub A] Receptor-Dependent Synchronization Leads to Ictogenesis in the Human Dysplastic Cortex
Quelle	In: Brain, 127 (2004) 7, S.1626-1640 (15 Seiten) PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0006-8950
Schlagwörter	Brain; Epilepsy; Seizures; Neurological Impairments; Pharmacology; Cytology + Suchen Sie Ihr Suchwort? Gehirn; Epilepsie; Anfallsleiden; Neurodegenerative Erkrankung; Arzneikunde; Zytologie
Abstract	Patients with Taylor's type focal cortical dysplasia (FCD) present with seizures that are often medically intractable. Here, we attempted to identify the cellular and pharmacological mechanisms responsible for this epileptogenic state by using field potential and K[superscript +]-selective recordings in neocortical slices obtained from epileptic patients with FCD and, for purposes of comparison, with mesial temporal lobe epilepsy (MTLE), an epileptic disorder that, at least in the neocortex, is not characterized by any obvious structural aberration of neuronal networks. Spontaneous epileptiform activity was induced in vitro by applying 4- aminopyridine (4AP)-containing medium. Under these conditions, we could identify in FCD slices a close temporal relationship between ictal activity onset and the occurrence of slow interictallike events that were mainly contributed by GABAA receptor activation. We also found that in FCD slices, pharmacological procedures capable of decreasing or increasing GABA[sub A] receptor function abolished or potentiated ictal discharges, respectively. In addition, the initiation of ictal events in FCD tissue coincided with the occurrence of GABA[sub A] receptor-dependent interictal events leading to [K(superscript +)][sub o] elevations that were larger than those seen during the interictal period. Finally, by testing the effects induced by baclofen on epileptiform events generated by FCD and MTLE slices, we discovered that the function of GABA[sub B] receptors (presumably located at presynaptic inhibitory terminals) was markedly decreased in FCD tissue. Thus, epileptiform synchronization leading to in vitro ictal activity in the human FCD tissue is initiated by a synchronizing mechanism that paradoxically relies on GABA[sub A] receptor activation causing sizeable increases in [K(superscript +)][sub o]. This mechanism may be facilitated by the decreased ability of GABA[sub B] receptors to control GABA release from interneuron terminals. (Author).
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Update	2017/4/10