Isothermal Titration Calorimetry and Macromolecular Visualization for the Interaction of Lysozyme and Its Inhibitors

Autor/inn/en	Wei, Chin-Chuan; Jensen, Drake; Boyle, Tiffany; O'Brien, Leah C.; De Meo, Cristina; Shabestary, Nahid; Eder, Douglas J.
Titel	Isothermal Titration Calorimetry and Macromolecular Visualization for the Interaction of Lysozyme and Its Inhibitors
Quelle	In: Journal of Chemical Education, 92 (2015) 9, S.1552-1556 (5 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/ed5002569
Schlagwörter	Undergraduate Students; Thermodynamics; Biochemistry; Science Laboratories; Laboratory Experiments; Science Experiments; Hands on Science; Science Instruction; Visualization; Computer Software; Correlation; Laboratory Equipment; Measurement Equipment + Suchen Sie Ihr Suchwort? Thermodynamik; Biochemie; Laboratory work; Laborarbeit; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Visualisation; Visualisierung; Korrelation; Laborausstattung; Messinstrument
Abstract	To provide a research-like experience to upper-division undergraduate students in a biochemistry teaching laboratory, isothermal titration calorimetry (ITC) is employed to determine the binding constants of lysozyme and its inhibitors, N-acetyl glucosamine trimer (NAG[subscript 3]) and monomer (NAG). The extremely weak binding of lysozyme/NAG is determined using a competitive binding assay. Such interactions between lysozyme and its inhibitors are visualized with PyMol software, by which the hydrogen bond formation in the complexes is used to explain the binding specificity. The hydrogen bond inventory in the binding interface correlates with the heat enthalpy determined or derived from ITC measurements. A possible explanation for such a correlation is presented and used for an extensive discussion in thermodynamics and ligand-receptor interactions. This laboratory exercise stimulates students' critical thinking about weak/strong binding interactions and the relationship between thermodynamics and structural changes. (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01